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Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
24/11/2016 |
Data da última atualização: |
23/10/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SILVA, P. R. A. da; VIDAL, M. S.; SOARES, C. de P.; POLESE, V.; ARAUJO, J. L. S. de; BALDANI, J. I. |
Afiliação: |
PAULA RENATA ALVES DA SILVA, UFRRJ; MARCIA SOARES VIDAL, CNPAB; CLEITON DE PAULA SOARES, BOLSISTA DA EMBRAPA AGROBIOLOGIA; VALERIA POLESE, UFRRJ; JEAN LUIZ SIMOES DE ARAUJO, CNPAB; JOSE IVO BALDANI, CNPAB. |
Título: |
Selection and evaluation of reference genes for RT-qPCR expression studies on Burkholderia tropica strain Ppe8, a sugarcane-associated diazotrophic bacterium grown with different carbon sources or sugarcane juice |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Antonie van Leeuwenhoek, v. 109, n. 111, p. 1493-1502, 2016. |
DOI: |
10.1007/s10482-016-0751-0 |
Idioma: |
Inglês |
Notas: |
Epub, 17 Aug., 2016. |
Conteúdo: |
Among the members of the genus Burkholderia, Burkholderia tropica has the ability to fix nitrogen and promote sugarcane plant growth as well as act as a biological control agent. There is little information about how this bacterium metabolizes
carbohydrates as well as those carbon sources found in the sugarcane juice that accumulates in stems during plant growth. Reverse transcription quantitative PCR (RT-qPCR) can be used to evaluate changes in gene expression during bacterial growth on different carbon sources. Here we tested the expression of six reference genes, lpxC, gyrB, recA, rpoA, rpoB, and rpoD, when cells were grown with glucose, fructose, sucrose, mannitol, aconitic acid, and sugarcane juice as carbon sources. The lpxC, gyrB, and recA were selected as the most stable reference genes based on geNorm and NormFinder software analyses. Validation of these three reference genes during strain Ppe8 growth on the same carbon sources showed that genes involved in
glycogen biosynthesis (glgA, glgB, glgC) and trehalose biosynthesis (treY and treZ) were highly expressed when Ppe8 was grown in aconitic acid relative to other carbon sources, while otsA expression (trehalose biosynthesis) was reduced with all carbon
sources. In addition, the expression level of the ORF_6066 (gluconolactonase) gene was reduced on sugarcane juice. The results confirmed the stability of the three selected reference genes (lpxC, gyrB, and recA) during the RT-qPCR and also their robustness
by evaluating the relative expression of genes involved in glycogen and trehalose biosynthesis when strain Ppe8 was grown on different carbon sources and sugarcane juice. MenosAmong the members of the genus Burkholderia, Burkholderia tropica has the ability to fix nitrogen and promote sugarcane plant growth as well as act as a biological control agent. There is little information about how this bacterium metabolizes
carbohydrates as well as those carbon sources found in the sugarcane juice that accumulates in stems during plant growth. Reverse transcription quantitative PCR (RT-qPCR) can be used to evaluate changes in gene expression during bacterial growth on different carbon sources. Here we tested the expression of six reference genes, lpxC, gyrB, recA, rpoA, rpoB, and rpoD, when cells were grown with glucose, fructose, sucrose, mannitol, aconitic acid, and sugarcane juice as carbon sources. The lpxC, gyrB, and recA were selected as the most stable reference genes based on geNorm and NormFinder software analyses. Validation of these three reference genes during strain Ppe8 growth on the same carbon sources showed that genes involved in
glycogen biosynthesis (glgA, glgB, glgC) and trehalose biosynthesis (treY and treZ) were highly expressed when Ppe8 was grown in aconitic acid relative to other carbon sources, while otsA expression (trehalose biosynthesis) was reduced with all carbon
sources. In addition, the expression level of the ORF_6066 (gluconolactonase) gene was reduced on sugarcane juice. The results confirmed the stability of the three selected reference genes (lpxC, gyrB, and recA) during the RT-qPCR and also their robustness
by evalua... Mostrar Tudo |
Palavras-Chave: |
Carbon metabolism; Diazotrophic bacteria; Normalizing genes; Real time PCR; Relative expression. |
Categoria do assunto: |
S Ciências Biológicas |
Marc: |
LEADER 02573naa a2200265 a 4500 001 2057134 005 2018-10-23 008 2016 bl --- 0-- u #d 024 7 $a10.1007/s10482-016-0751-0$2DOI 100 1 $aSILVA, P. R. A. da 245 $aSelection and evaluation of reference genes for RT-qPCR expression studies on Burkholderia tropica strain Ppe8, a sugarcane-associated diazotrophic bacterium grown with different carbon sources or sugarcane juice 260 $c2016 500 $aEpub, 17 Aug., 2016. 520 $aAmong the members of the genus Burkholderia, Burkholderia tropica has the ability to fix nitrogen and promote sugarcane plant growth as well as act as a biological control agent. There is little information about how this bacterium metabolizes carbohydrates as well as those carbon sources found in the sugarcane juice that accumulates in stems during plant growth. Reverse transcription quantitative PCR (RT-qPCR) can be used to evaluate changes in gene expression during bacterial growth on different carbon sources. Here we tested the expression of six reference genes, lpxC, gyrB, recA, rpoA, rpoB, and rpoD, when cells were grown with glucose, fructose, sucrose, mannitol, aconitic acid, and sugarcane juice as carbon sources. The lpxC, gyrB, and recA were selected as the most stable reference genes based on geNorm and NormFinder software analyses. Validation of these three reference genes during strain Ppe8 growth on the same carbon sources showed that genes involved in glycogen biosynthesis (glgA, glgB, glgC) and trehalose biosynthesis (treY and treZ) were highly expressed when Ppe8 was grown in aconitic acid relative to other carbon sources, while otsA expression (trehalose biosynthesis) was reduced with all carbon sources. In addition, the expression level of the ORF_6066 (gluconolactonase) gene was reduced on sugarcane juice. The results confirmed the stability of the three selected reference genes (lpxC, gyrB, and recA) during the RT-qPCR and also their robustness by evaluating the relative expression of genes involved in glycogen and trehalose biosynthesis when strain Ppe8 was grown on different carbon sources and sugarcane juice. 653 $aCarbon metabolism 653 $aDiazotrophic bacteria 653 $aNormalizing genes 653 $aReal time PCR 653 $aRelative expression 700 1 $aVIDAL, M. S. 700 1 $aSOARES, C. de P. 700 1 $aPOLESE, V. 700 1 $aARAUJO, J. L. S. de 700 1 $aBALDANI, J. I. 773 $tAntonie van Leeuwenhoek$gv. 109, n. 111, p. 1493-1502, 2016.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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Registro Completo
Biblioteca(s): |
Embrapa Unidades Centrais. |
Data corrente: |
13/05/1993 |
Data da última atualização: |
02/08/2011 |
Autoria: |
AVILA, A. F. D.; CAMPOS, F. |
Afiliação: |
EMBRAPA-DTC. |
Título: |
O planejamento da pesquisa agropecuaria na EMBRAPA. |
Ano de publicação: |
1985 |
Fonte/Imprenta: |
Brasilia, DF: EMBRAPA-DTC, 1985. |
Páginas: |
20 p. |
Idioma: |
Português |
Notas: |
Versao preliminar. |
Palavras-Chave: |
Agropecuaria. |
Thesagro: |
Pesquisa; Planejamento. |
Thesaurus NAL: |
agriculture; planning; research. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00515nam a2200205 a 4500 001 1094649 005 2011-08-02 008 1985 bl uuuu u00u1 u #d 100 1 $aAVILA, A. F. D. 245 $aO planejamento da pesquisa agropecuaria na EMBRAPA. 260 $aBrasilia, DF: EMBRAPA-DTC$c1985 300 $a20 p. 500 $aVersao preliminar. 650 $aagriculture 650 $aplanning 650 $aresearch 650 $aPesquisa 650 $aPlanejamento 653 $aAgropecuaria 700 1 $aCAMPOS, F.
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